Generally speaking, leaves are quite small, but collectively they present a massive surface area to the environment.  The total surface of leaves is significantly greater than the surface area of the Earth.  Leaves are the organs of photosynthesis, not only do they make the basic chemicals for the plant, but they replenish the oxygen in the atmosphere. Generally speaking, a leaf has two surfaces.  One surface (the adaxial) is presented to the sun, the other (the abaxial surface) is shielded from the light to some extent.  Both surfaces are covered with a waxy layer (the cuticle), which helps restrict water loss. The underside of the leaf has many tiny pores, the stomates, which can open and close to allow the exchange of gases and water vapour.  The lower surface may be covered in minute 'hairs'.  These create an unstirred layer, so that air next to leaf has a high water content and thus water loss through the stomates is reduced.  The veins of a leaf contain xylem tissue which delivers water and mineral salts, and phloem which transports sugars and other organic compounds from the leaf. [caption id="attachment_43706" align="alignleft" width="300"] adaxial surface[/caption] [caption id="attachment_43705" align="alignright" width="300"] abaxial surface[/caption]   The upper  surface and lower surface  of a foxglove leaf.  Whilst the veins can be seen on the top surface, they are ridged and covered in hairs on the lower surface. [caption id="attachment_13486" align="alignleft" width="240"] galls on sycamore[/caption]   Once a leaf has grown then its size, shape and the veins remain constant, but during the course of the year other things can and will change.  For example, in autumn the coloured pigments in the leaf break down and many compounds are transported out of the leaf as it undergoes senescence. The leaf may be subjected to attack, caterpillars and leaf miners may eat its tissues.  Certain wasps may lay eggs within the leaf so that a gall develops.  Greenfly use their piercing mouthparts to access sugars and other compounds from the phloem.  Bacteria may infect the leaf either through wounds inflicted on the leaf, or by penetrating the stomates. To some micro-organisms, a leaf is ‘home’; it is a habitat.  Rather like it is now common to talk about our gut microbiome or  the microbiome of the skin, so each leaf has a microbiome. Recently, microbiologists from China and Germany have studied the microbiome of oak leaves.  Specifically, they examined the microbiome of the leaves of the pedunculate oak.  They examined how the organisms present changed during the course of a growing season.  They found that the upper and lower sides of the leaves had different communities of micro-organisms.  Both surfaces  shared the same sorts of bacteria, but they varied in the amounts / proportions of the bacteria on the two surfaces.  Furthermore the differences between the upper and lower surfaces changed as the seasons changed.  The diversity of organisms on the lower surface of the leaf fell as the year progressed.  Some of the bacterial DNA on the lower surface was that of bacteria which could be found on / in aphids and whitefly.  Some of the bacteria found were associated with fermentation of sugars.   The study did not investigate fungal species on the leaves, but it does indicate that there are complex microbial communities present on leaves, and that the two surfaces offer different ‘opportunities’ for micro-organisms.  Similarly, it has been established that there is a root microbiome. The root microbiome is a complex community of bacteria, fungi, archaea, and viruses living in and around plant roots. This microbiome helps the plant take in nutrients, fight diseases, and cope with environmental stress. [caption id="attachment_43724" align="alignright" width="350"] Hornbeam - abaxial surface[/caption]   [caption id="attachment_43723" align="alignleft" width="350"] Hornbeam - adaxial surface[/caption]

The grey—brown skeletal branches of trees are being cloaked in fresh green leaves as they unfurl from the buds that protected them through the winter months.  Their bright green colour is due to large amounts of chlorophyll.  The chlorophylls are pigments that can absorb many of the wavelengths of visible light, but not green.  Green wavelengths are reflected back into the environment, which is why our eyes perceive both young and mature leaves as green. Each leaf is made up of a variety of cells and tissues.  The top and bottom of the leaf are covered with a layer of cells  termed the epidermis.  It consists of many interlocking cells (rather like jigsaw pieces), sometimes called pavement cells.  Their function is to protect the underlying cells and also produce the waxy, waterproofing layer — the cuticle.   The lower epidermis is ‘pierced’ by the stomates.  These are the ‘breathing pores’ of the leaf, allowing the exchange of gases and water vapour.   The epidermis may also bear trichomes.  These are small ‘hair—like’ projections.  If there are many of them they can give the leaf a white or silvery appearance, helping to trap moist air near to the leaf surface to reduce water loss.  They  may also help to reflect sunlight, so that the leaf does not get too hot and on cold days can serve to protect the leaf from frost damage. Some trichomes have a protective function in that they may physically restrict the feeding of insects and other herbivores, and some contain a cocktail of toxic chemicals [e.g. nettles]. Under the upper epidermis and within the leaf, there is one or more layers of cells packed with chloroplasts - the palisade layer.  This is the principal site of photosynthesis within the leaf, where carbon dioxide is fixed into sugars and other vital nutrients.  The ‘by-product’ of photosynthesis is oxygen, which is not only essential for plant respiration but needed by the vast majority of animals on this planet.  It diffuses out of the leaf through the intercellular spaces of the next layer of the leaf - the mesophyll layer. The stomates allow gases in and out, but can close through the movement of their guard cells.  Stomates tend to close up at night or when the leaf experiences water stress.  Running throughout the body of the leaf is the xylem and phloem tissues, which conduct water, minerals and sugars etc around the plant. The sheer abundance of chlorophyll in many leaves masks the presence of other pigments, which only become visible when the leaf begins to senesce and the chlorophylls break down.  The leaf turns a yellow / orange colour due to the presence of carotenoid pigments.  Autumnal leaves can display a variety of colours due to other pigments such as the anthocyanins and xanthophylls.  Some leaves take protection very seriously   Curious fact : the leaf with the largest surface area is that of the Amazonian water lily, which can be 10 feet in diameter.

City temperatures are rising across the Earth.  Heat stress causes illness and even death. Between March and June last year, India experienced record-breaking temperatures, with 37 cities surpassing 45oC. Hundreds of people died from heat death and over 40,000 heatstroke cases in 17 states were recorded.  Increasing urban temperatures also mean greater use of air conditioning (and associated energy use).  In an attempt to mitigate these rising temperatures, many cities have implemented tree planting programmes, e.g. One Million Tree campaigns. During the day, trees cool cities by: Blocking the sunlight from reaching certain areas The loss of water vapour (transpiration) via their stomates - tiny pores on the underside of leaves Changing the flow of air in and around trees and buildings. These are the positive aspects of trees in cities, but there can be a downside to their presence. The heat build-up  stored in roads and buildings during the day can irradiate back into the atmosphere at night, but it can be ‘trapped’ by a dense leaf tree canopy. Cambridge researchers have now analysed the results of some 182 recent studies of trees in different cities and countries / climates, and have reported that : Urban trees can result in an air temperature reduction of some 12oC as experienced at the pedestrian level Trees reduced the peak monthly temperatures in most of the cities studied The cooling effect varied significantly, according to the tree species present, the urban layout and the climate the trees experience. Seemingly city trees exert a greater cooling effect in hot and dry climates, and less in hot and humid climates.  For example, in tropical rainforest climates [which experience very high humidity] then the day time cooling effect may be as low as 2oC, and the warming effect at night +0.8oC.  The study also noted that cities with an ‘open’ urban layout with a mix of evergreen and deciduous trees (of different sizes) were more likely to experience a good cooling effect.  However, in cities with a dense or compact layout, such as Cairo or Dubai, planting mainly evergreen species was found to be more effective. The study concludes that whilst urban planners need to provide more green space in cities,  they must also carefully consider the ‘mix’ of trees that are planted.  The study also emphasises that trees alone will not provide a solution to rising temperatures, but the use of solar shading and reflective materials will play a part, as will the nature and structure of the urban layout. Full details of this study can be found here :  https://www.nature.com/articles/s43247-024-01908-4  

During a drought, the trees in a woodland or forest become 'stressed' and may die.  The  reason for their death is not immediately obvious (beyond lack of water), and  it is not possible to ‘transplant’ a mature tree and its complete root system to a lab for detailed investigations.  However, recently, researchers at the University of Innsbruck have taken ‘the lab’ to a set of mature pine and pine trees. The trees were fitted with rugged and waterproof ultra-sound detectors.  Some of the trees had their canopies covered by a ‘roof’ so that the summer rain was denied to the trees, and they essentially experienced a ‘drought’.   Drought stressed trees produce ultrasound ‘clicks’ (faint acoustic waves that bounce off of air bubbles) that can be picked up by the detectors.  Air bubbles or emboli form in the vascular system of the trees when they are struggling for water.  Water is drawn up the xylem vessels by the evaporation of water (via the stomata) from the leaves, there is a continuous column of water.  When the column of water breaks, bubbles form with the xylem vessels and the transport of water to the leaves is reduced.  If the flow of water is substantially reduced the tree will die. The sound detectors found that the spruces produced more clicks than the beeches when water stressed, suggesting more emboli were formed within their xylem tissues.  It may be that the beeches were able to access the deeper reserves of water in the soil, whereas the spruces had a shallower root system. Trees can, of course, reduce water loss from their leaves by closing down their stomates.  But when their stomates are closed, they cannot take in carbon dioxide for photosynthesis and make the sugars / starch that they need for their metabolism.  At the end of the experiment, the trees that experienced ‘drought’ were drenched with water and most recovered well, and their rates of photosynthesis caught up with the ‘control’ groups of trees (those with summer rain).  However, the spruces’ water reserves were somewhat depleted; this was determined by measuring the resistance the tissues offered to an electrical current. The ability to withstand / recover from drought could over time affect the make up of woodlands and forests,  particularly if the trend for hotter and drier summers continues. Interestingly, some work in the United States (at University of Wisconsin–Madison) suggests that young tree saplings that have experienced drought or heat are more likely to survive when transplanted into more challenging areas.  It seems that the soil microbes that young saplings experience can help young trees establish themselves.  Saplings grown in soil (and microbes) that have experienced drought / cold / heat are more likely to survive when later transplanted and faced with similar conditions.  Trees with ‘cold-adapted’ microbes survived better when experiencing Wisconsin’s winter temperatures. The work was conducted with different species of tree in a variety of locations in Wisconsin and Illinois. The transplant locations varied in temperature and rainfall.  It may be that fungi that inhabit the roots of the saplings are involved in these ‘responses’, though the microbial population of the soil is diverse. For more details of this work, follow the link here.

The olive tree [Olea europaea] , is an evergreen tree native to the mediterranean area.  Indeed, it is often cited as an ‘indicator plant’ for the area. It is of major agricultural importance throughout the mediterranean basin as it is the source of olive oil.  Generally, the outline of the tree is somewhat short and squat†, its height varies between 8 –15 M. The oblong/spear-shaped leaves are silvery green; the upper surface is green, whereas the lower surface is more silvery. The leaves measure between 4–10 cm in length and are between 1–3 cm in width. Olive trees can be very long lived and, as the tree ages, the trunk frequently becomes gnarled and twisted.  The tree produces small, white, ‘feathery’ flowers on the previous year's growth. Read more...

In 1880, Darwin wrote a book entitled “The power of movement in Plants” in which he described many examples of the “sleep movement’ of plants.  He noted that some flowers closed up at night (e.g. crocuses, tulips, poppies), whilst other plants had leaves that moved or closed up at night time.  He recorded that the pea and bean family (legumes / fabaceae) had a number of species that exhibited these ‘sleep movements”. Movement in plants is achieved either through growth or through changes in the water content of cells (the turgidity of cells).  Phototropism is an example of an irreversible growth response Read more...

According the United Nations FAO, some two million hectares of forest were burnt in the Mediterranean region, between 2006 and 2010.  Most of these fires were 'human induced'; they (the fires) are the most frequent cause of degradation / loss of forest / woodland in this region.  In 2012, a fire swept through some 20,000 hectares of forest near Andilla (Valencia).   After the fire, it was found that though oaks, holm oaks, pines and junipers had been completely burnt, the vast majority (98% +) of the Mediterranean Cypresses were still standing, tall and green.   There followed a three year study of the fire resilience of the Mediterranean Cypress (Cupressus sempervirens var horizontalis) to see if stands of these trees might function as buffer zones to hinder or prevent the spread of forest fires. Read more...